Lady Anabelle's unexpected death done by Jowy, the main character's best friend in an act of "betrayal".
When your sister Nanami falls (whether she dies or not is up to whether you recruited all 108 stars), it is a huge Tear Jerker moment, especially when she tells the hero how happy she was to be his "Big Sister". Also, Pilika's plotline is a huge Tear Jerker. She loses her village and her parents, is rendered mute when she is nearly butchered by Luca Blight, is apart from her beloved caretaker Jowy for a long time, and in the end, there is a scene where Jowy tells Pilika than when he leaves, its "goodbye forever". Pilika tells Jowy to hold her like her father held her.