This is based on opinion. Please don't list it on a work's trope example list.
Tear Jerker / Resident Evil: Operation Raccoon City
In "Operation Raccoon City", near the end of the the game you have the choice to either kill or protect Leon. Unless all four people choose one option, you end up killing the other members of your team. What's truly heartbreaking is throughout it all, both sides are trying to convince the other to come over to their side. Despite all the friction and animosity shown, these pieces of dialougue show that they all cared for each other and having to fight each other to the death is really heartrending for them