The penultimate conversation with the AI Adam was an even bigger Tear Jerker:
Adam: Did this "Adam" care for you? Would he sit in a safe command room somewhere and order you to die?
Samus: He would understand that some must live and some must die... He knew what it meant. He made that sacrifice, once.
Adam: So he chose life for you? Our fair warrior? Your Adam gave his life so that you might keep yours... for the sake of the universe...
Samus: (long pause)
Adam: Samus, this is your last mission. Go to the Operations Room and alter the station's orbit path to intercept SR388. Set the self-destruct, return to your ship and escape. Move quickly, and stay alive... Any objections, Lady?