In Shiri, the main characters go to see a Korean version of the musical.
In Tootsie, a drunk John waits outside Dorothy's apartment for her when she comes home. When she refuses to let him, John sings, "I'll Know" on the street until Dorothy lets him in.
Julie & Julia features the song "A Bushel and a Peck" during a montage scene.
Oh, God!: The third film in the trilogy Oh God! You Devil! starts and ends with a father praying over a sick child and singing for "Fugue for Tinhorns".
In an episode of Counterpart (2018), Clare, or, rather, her double, sings "A Bushel and a Peck" to her baby girl to quiet her down.
An episode of Good Luck Charlie is titled "Guys and Dolls". In this episode, Bob tries to give Teddy advice on how to make up with Spencer when the two have a disagreement, which backfires immensely, while Amy tries to get Charlie to like Toby, her baby brother, by having her play with a baby doll that looks like him.
Sanders Sides: In "Moving On Part 1/2" and "Moving On Part 2/2", Thomas gets wistful about an ex-boyfriend he met while doing a production of Guys and Dolls. Just the name of the play is a Trauma Button for the other Sides. Roman also manages to slip in a Take That! about it during an otherwise serious scene, due to its perceived Values Dissonance. Provides a particularly clever play on words:
The Simpsons: In "Mayored To The Mob", Mark Hamill stars in a Springfield production of Guys and Dolls, which has hastily received a Star Wars-themed retool, with Hamill singing "Luke, Be A Jedi Tonight" instead of "Luck Be A Lady Tonight".