- General Posada's men are a quartet of skinny, wimpy fellows (one of whom is a leper who keeps losing his arms), and they get a lot of funny moments, like when they keep getting beat up by a young Joaquin.
- Manolo's mariachi friends try to help him serenade Maria with impromptu renditions of increasingly inappropriate songs, such as "Just a Friend" by Biz Markie. The result? Increasingly large cacti lobbed at their heads by Chuy.
- Pablo, the smallest of the group, starts up his turn of romantic wooing songs. Starts counting down aaaand...Cue giant potted cactus.
- For bonus points: that countdown is the intro to "Woolie Bully". Not the most romantic choice.
- Joaquin giving Maria an autographed photo of himself as a present, as well as her reaction.
- Just before that, while describing his medals:
Joaquin: "And this is the medal for most medals."
- The Human Ladder Manolo and the Rodriguez brothers form to reach Maria's balcony toppling over when she stops his kiss.
Maria: Did you think it was gonna be that easy?
(Manolo and friends topple over)
Manolo: (dizzy) I…I kinda did…
- All three mariachis landing on Manolo, along with their instruments.
- Joaquin and Manolo's argument over Maria initially seems serious, with the two of them throwing very low blows at each other. Until it turns into two grown men pointing angrily at each other and then having a slap fight.
Joaquin: So childish!
- Every time Joaquin calls out his own name during an action scene.
- The fact that he has theme music for said action scenes.
- The reason why Manolo's great-grandmother joins the rest of the Sanchez family just as Manolo fulfills his wager with Xibalba:
Grandma: Eh. Cholesterol.
- The fact that members of the Sanchez family keep dying and immediately popping up in the Land of the Remembered as this scene goes on. It is Black Comedy, but funny to see everyone's casual reactions towards death.
- The way Xibalba's jaw drops when Manolo wins their wager.
- Manolo emerges completely unscathed from the explosion under the bell. Everyone, including Xibalba, La Muerte, and the Candlemaker, are confused and Manolo turns to La Muerte. Her response?
La Muerte: Don't look at me.
- Nearly everything the Candle Maker does.
- When La Muerte finds out that Xibalba cheated at their bet, she screams his name and he appears right away - with a bottle of wine and two glasses, clearly with no idea of just how much trouble he's in. Did the absolute fury not give it away?
- The big fight between the citizens of San Angel and Chakal's army of bandits has some hilarious moments:
- Before the fighting starts, Joaquin tells Chakal to pick on someone his own size, at which point we get a full view of the scene, Chakal TOWERING over Joaquin, and someone screams:
Town Citizen: Cause no one's that big, man!
- The priest dressed as a luchador. Especially when he briefly gets his hands on the Medal of Everlasting Life and becomes virtually unstoppable.
- Chuy becoming the leader of a pig army.
- After Manolo's kill-less fight with a bull causes the town to ridicule and shame him, there's actually a pretty funny shot where even the bull is sad and disappointed with him.
- Goth Kid's reaction to hearing about Maria's apparent death:
- And shortly after, his reaction to Manolo being killed by Xibalba's two-headed snake staff:
- Even funnier when you learn that Goth Kid is a Mexican according to the art book.
- The Goth Kid calling both La Muerte and Xibalba beautiful in an awestruck tone.
- When Carlos mentions that all of the Sanchezes have been bullfighters, it apparently included his grandmother, judging from her comment right afterwards.
Grandma: I was a beast in the arena. A beast!.
- Carlos expresses his exasperation that Manolo doesn't kill the bulls he fights, then one of the mariachi brothers helpfully says that he did the other day. Flashback to Manolo bullfighting when the bull is suddenly struck by lightning and flops over dead, Manolo getting a "WTF?" expression.
Grandma: That didn't count.
- Everyone's reaction to Maria when she returns to San Angel after several years studying abroad. Even Xibalba was shocked by how beautiful she was, much to the annoyance of La Muerte, who was standing right next to him at the time!
Xibabla: (Has a bug-eyed "Holy crap, she's HOT" look on his face) La Muerte: (smacks him with an annoyed look on her face) Xibalba:
- La Muerte doing a Coy, Girlish Flirt Pose to Xibalba in order to get the latter to return Manolo back to life. He catches on and uses one of his wings to cover her. La Muerte moves them aside and begins to rubs his feathered wing. And asks in a seductive, pleading tone, "Please? Balby?"
Manolo, Carmen, Luis, and Candle Maker: (In unison) "Balby"?!
- During the dinner scene when Joaquin puts his foot in his mouth by making a sexist comment that insults Maria, one of the nuns sitting at a nearby table picks up a knife and shoots a Death Glare in Joaquin's direction, while her fellow sisters begin to look incredibly nervous.
- "Ooooo! You picked yourself a feisty one! Ha-" (gets punched)
- The little boy selling churros in the beginning.
Boy: Churros! Get your churros here! (nearby bird poops on them and there's a pause) Frosted churros!