This is an index for the Mystery Incorporated versions of the characters in this Scooby-Doo series, which are different in personality than longtime fans are familiar with. Huge spoilers ahead.
- Mystery Incorporatednote Scooby-Doo, Shaggy Rogers, Velma Dinkley, Fred Jones, Daphne Blake
- Antagonistsnote Fred Jones Sr., Professor Pericles, Mr. E, Ed Machine, Brad Chiles and Judy Reeves, the Nibiru Entity, Alice May, Danny Darrow, Crybaby Clown, George Avocados, Gary and Ethan, various monsters, Kriegstaffelbots
- Other Charactersnote Angel Dynamite, Sheriff Bronson Stone, Skipper Shelton, Vincent Van Ghoul, H.P. Hatecraft, Marcie "Hot Dog Water" Fleach, Janet Nettles, Angie Dinkley, Nova, other parents
