Trivia: Professor Layton and the Eternal Diva
- Hey, It's That Voice!: Chris Miller and Maria Darling reprise their roles as Layton and Luke from the games, and they are joined by several actors from Dragon Quest VIII.
- The Other Darrin: Dr. Schrader, Flora and Don Paolo, during their guest appearances at the beginning, have different voices from the games. It's understandable with Flora, since Lani Minella is a U.S.-based actress and the movie was dubbed in the United Kingdom, but it is somewhat puzzling why Don Paolo has a different actor, since Chris Miller voiced both Layton and Don Paolo.
- Sequel First: In the UK, where it came out before Professor Layton and the Last Specter. Averted in the US, where the delayed release meant Last Specter got out first (just barely, as Eternal Diva came less than a month after).