- Actor Allusion: Several times. Casey's Frasier references (she's Kelsey Grammer's daughter); Ferris Bueller references in front of the Dean (Cameron Frye from that movie). Abby(played by Olesya Rulin) saying she choreographed 3 high school musicals and the third one being their senior year, shortly followed with Casey saying "we're all in this together".
- Contest Winner Cameo: One of the judges in "Mr. Purr-fect".
- Hey, It's That Guy!: Lesse, aside from Cameron Frye being the dean and Cordelia and Delia Fisher being ZBZ consultants, there's Jerry Lambert ("...well played, Mauer") as Casey and Rusty's dad, and Dwayne Wayne as Ashleigh's dad, Leah Thompson as Cappie's mom...
- Caroline Forbes really shouldn't be allowed to take care of animals.
- Sanjay apparently transferred to Greendale Community College.
- Could Steve's last name be Holt?
- The ZBZ National President? Babs from Animal House, naturally.
- The Plain White Ts appear in several episodes and in Season 2, it's revealed that they're part of Kappa Tau.
- Betsy is Kyla Woods
- Hey, It's That Voice!: Homer Simpson as Rusty's physics professor.
- Retroactive Recognition: Travis and Kirsten as Dale's old friend from home and Frannie's big sister Kiki respectively. Also Mike Chang was a background Omega Chi and Naomi and Adrianna were Cappie's mystery girl and Rebecca's fellow ZBZ pledge who ended up in the fountain.
- Romance on the Set: Amber Stevens (Ashley) and Andrew West (Fisher) began dating on set and later married, even though their characters didn't last.
- What Might Have Been: Scott Michael Foster (Cappie) originally auditioned for the role of Calvin note , Jake McDorman (Evan) auditioned for Cappie, Clark Duke (Dale) auditioned for Rusty, Jacob Zachar (Rusty) auditioned for Dale, and Dilshad Vadsaria (Rebecca) auditioned for Ashleigh.
Clark Duke: "So thank god we got that sorted out in time."