- Acting for Two: Bobby Moynihan voices both the Penguin and Bat-Mite.
- Full Circle Portraying: Between this and The Batman (2022), Jeffrey Wright is the second person to play Batman himself and Commissioner Gordon (the first being Benjamin McKenzie),
- The Other Darrin: During her brief appearance in season 1, Harley was voiced by Heidi Gardner. Season 2 sees Harley voiced by Gillian Jacobs.
- Role Reprise:
- Brent Spiner reprises his role as The Joker from Young Justice.
- Season 2 sees Gillian Jacobs reprise her Injustice (2021) role as Harley Quinn.
- Colorblind Casting:
- Quite possibly the first time that The Riddler has been played by an actor of color.
- Far from the first time for Batman himself (given Anthony Ruivivar, who voiced him on Beware the Batman, is Filipino/Chinese/Scottish/German and both Kevin Michael Richardson, who voiced Batman is some DC Nation shorts, and Keith Ferguson, who voiced him in DC Super Hero Girls 2019, are African-American), Robin (given DC Super Hero Girls 2019 also cast Keith Ferguson as Dick) Catwoman, or Commissioner Gordon (having been played by Jeffrey Wright in The Batman (2022)).
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