And Peter's reaction to his death is heartbreaking. He's screaming for someone to call an ambulance, and plainly has no idea what to do when he's attempting to stop the bleeding.
Gwen showing up at Peter's house after her father's funeral and desperately asking him why he wasn't there.
Everything Aunt May goes through, her family slowly being ripped apart first with the Parkers disappearing, then her husband being shot, and her nephew that she raised coming home late at night covered in cuts and bruises. And then at the end of film, he comes home covered in blood and she just holds him tightly.