- Hirohiko Araki is a particularly big fan of Nelly, and included a lot of homages to him in JoJo's Bizarre Adventure: Steel Ball Run.
- The horse of Dot Han is named #1, after the Nelly song of the same name.
- One of the Steel Ball Run race participants is literally named Nellyville, after the album and song, while his horse is named Country Grammar, after the Nelly album of the same name.
- In Madagascar 3: Europe's Most Wanted, after the Penguins' plane crashes in the outskirts of Monaco, King Julien is seen getting electrocuted atop a high tension wire, weakly singing an impromptu version of Nelly's "Hot in Herre".
- In Turning Red, Tyler's bandaid and headband look is a reference to Nelly during his peak years.
- In an early episode of Glee, the entire glee club sings "Ride Wit Me"
.
Music
- "Weird Al" Yankovic's "Trash Day" is a parody of "Hot in Herre."
- Hark! A Vagrant: "Founding Fathers (In a Mall)" the founding fathers are a bit panicked by "Hot in Herre", declaring that a window needs to be opened.
- DashieXP: The titular character of the "Ghetto News Anchor" video quotes the lyrics to "Hot in Herre" while reporting on a heat wave.
- Family Guy: One Cutaway Gag has Stewie beat up Justin Timberlake for his award and giving it to Nelly instead.
- My Little Pony: Friendship Is Magic: When Pinkie Pie gets a rap number, one of her backup dancers is dressed like Nelly.
