- The One Piece character Franky (a.k.a. Cutty Flam) was based on Ace, at the admission of One Piece creator Eiichiro Oda.
Films - Live-Action
- Billy from Most Likely to Murder (2018) has an Ace Ventura: Pet Detective poster in his childhood bedroom. He does Jim Carrey impressions in several scenes.
- Sister, Sister had Tamara frequently use Ace's "Aaaaaaaaallll-righty then" Catchphrase.
- World of Warcraft has a minor gnome adventurer NPC called Finkle Einhorn, named after Ray Finkle and Lois Einhorn.
- How It Should Have Ended: In "How Sonic the Hedgehog Should Have Ended", Robotnik summons an army of robots based on various characters that Jim Carrey has played over the years, including Ace Ventura. The latter is destroyed by Shadow the Hedgehog, who even says "Alrighty then." before the finishing blow.
- The Simpsons: In "Yokel Chords", the song "Cultural Things Experience" has the lyric "Comparing Jim Carrey to Dario Fo!", with Carrey appearing as Ace Ventura.