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Anime and Manga
- In Afro Samurai, the young protagonist takes this expression very shortly after seeing his parents killed. This is the moment he is truly destined to become a badass.
- Vash (above) looks like this whenever he drops his facade of idiocy and decides to get serious. Only to be expected from a man whose byword is determination.
- Tengen Toppa Gurren Lagann takes this trope Up to Eleven in typical style. These happen, of course, throughout the series, but the crowner comes in the grand finale, when a stunning "The Reason You Suck" Speech from the Big Bad is countered by Determined Looks from people across the globe, as they send a Combined Energy Attack to the titular mech and simultaneously kick off a Last Episode Theme Reprise.
- The extremely badass Ronin Nanashi from Sword of the Stranger. When he gets that I-will-kill-you expression◊ on his face, you know you're fucked.
- In Soul Eater, there's a shot of each of the main characters displaying one in turn just before the first battle for the B.R.E.W.
- Yu-Gi-Oh!: Kaiba is the master of this trope. Also, if Yami Yugi does this instead of making a speech, things are about to get intense. The gang has been known to call this his "game face". For Joey, this is usually the signal that you've managed to flip his switch from Plucky Comic Relief to Let's Get Dangerous!. Crowning moments of awesome never fail to ensue.
- Goku, from Dragon Ball has this right after turning Super Saiyan for the first time, except his arms are akimbo.
- Goku also sports this look right before he fights Vegeta in the Saiyan saga.
- Whenever somebody gives him the "Because Destiny Says So" speech or the "live to fight another day" speech when he's faced with a difficult opponent, Guts from Berserk always gets this look. A very memorable moment was when Guts faced Griffith for the second time in order to win his freedom from the Band of the Hawk.
- The original Mazinger Z Humongous Mecha is famous for being able to adopt this expression at will (remarkable feat in a robot without movile facial features) through subtle (for the 1970's) camera angles.
- Elseworld's Finest: Supergirl & Batgirl: Supergirl wears this expression -brow furrowed, eyes narrowed, lips tight, body tense and expression furious and deadly serious- when she confronts Lex Luthor after discovering that he murdered her cousin Superman.
- Convergence: The Adventures of Superman #1. After seeing visions foretelling her death, pre-Crisis Supergirl becomes distressed for several short seconds before deciding that it is irrelevant whether she dies or survives: she has to save them all. And she wears a downright determined expression as she states this◊.
- Superman: Brainiac: After Superman tells Kara that she has to stop a missile and he trusts her, Supergirl's expression goes from fright and shock to steely determination, eyes narrowed, brows furrowed and lips tight◊.
- In The Belgariad and The Malloreon, this notably graces the faces of the likes of Eriond and Durnik when they do important things, such as desecrating a sacrificial altar and banishing a demon back to the underworld.
Live Action TV
- At the end of de Blob 2, the normally cheerful and carefree Blob gets quite serious after Comrade Black uses a giant hypno-disc to hypnotise the planet. He gives Comrade Black's surveillance camera a look which very clearly says, "I am coming for you, and you will pay for this".
- In Super Robot Wars, this is pretty much Kyosuke Nanbu's default expression.
- Auron's (second) introduction in Final Fantasy X. As panicked civilians are running from a big-ass lizard, he just looks at it and gets ready to kill it.