When Kikuchiyo tries to prove his lineage with a scroll. As the senior members of the Seven examine it, you can see in the background one of the other members count on his fingers the years involved in Kikuchiyo's claim. He begins to laugh uproariously in quiet just before the readers realize that Kikuchiyo would have to be a child for his evidence on the scroll to make sense.
Kikuchiyo demonstrates his skill as a horse rider by riding behind some bushes... The horse comes gallops out alone with Kikuchiyo chasing it.
Kambei breaking the tension before the final battle with the bandits by telling Katsushiro, who was discovered with one of the farmer's daughters the night before to "Fight bravely, for now you are a full fledged man!" causing both the farmers and the other samurai to laugh.