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Moon Knight's The Comically Serious type personality makes for a few laughs in his appearance in Spider-Man: Web of Shadows. Especially the part with him and Vulture arguing over whether or not freeing the Tinkerer would be in Spidey's interest. Eventually the Web-Head gets annoyed, shouts at the two, and asks which one would prefer to fly him there. Moony just slowly lifts his hand up like a child.
Another happens in his interactions with Spider-Man beforehand, where he questions why Spider-Man doesn't simply destroy Wilson Fisk. He sees no problemin this.
In the 2014 Warren Ellis series, the first issue has this, while Spector is in his Mister Knight persona for the first time on panel: