Moon Knight's The Comically Serious type personality makes for a few laughs in his appearance in Spider-Man: Web of Shadows. Especially the part with him and Vulture arguing over whether or not freeing the Tinkerer would be in Spidey's interest. Eventually the Web-Head gets annoyed, shouts at the two, and asks which one would prefer to fly him there. Moony just slowly lifts his hand up like a child.
Another happens in his interactions with Spider-Man beforehand, where he questions why Spider-Man doesn't simply destroy Wilson Fisk. He sees no problemin this.