In the first game, some of the commentary from the Delta operators, particularly Holiday, is hilarious.
Holiday: (disarming explosives) Man, whoever rigged this was definitely a pro. Bishop: Is that supposed to make me feel better? Holiday: He may be a pro, but I'm a master.
Betters has plenty of commentary to add over the course of the mission, like when dealing with Alice Wade:
Betters: Those soldiers are after you. If they capture you again, they're going to interrogate you - and believe me, their methods are very unpleasant. Alice Wade: I'll take my chances. Betters: Stubborn little bitch, isn't she?
Later, when Jin and the Point Man meet back up:
Jin: Thank God you're alright! Betters: You two can make out later, now get on the chopper!
Alice freaking out if the Point Man decides to leave the elevator to take the fight to the Replica.
Alice: You don't have to kill everybody!
Really, the entire escort sequence. Between the incongruously cheery (and incredibly irritating) music in the elevator to Alice's increasingly horrified reactions to the Point Man getting his Blood Knight on, it's one of the most hilarious moments in the game.
When it comes to unscripted moments, smacking an enemy while in Slow-Mo will produce a hilarious bobblehead effect. You can almost imagine a Looney Tunes-esque boing noise whenever their heads start bouncing back and forth.
Except for a very small amount of static objects, almost every corpse in the game can be thrown about by melee. Since they're almost always on the ground, the easiest method to reach them is the slide-kick... and using that will fling then twenty feet away.
Speaking of smacking people around, the three Replica soldiers after you pop Fettel's head. Walk up, bitchslap, repeat.
One of the signs found throughout the warehouse and Armacham Lab:
Remember It's: Quantity Quality Safety IN THAT ORDER
There's also a "Help Keep This Room Clean" sign in a room covered in at least two humans worth of blood coming from the vent above.
The second time you meet Norton Mapes he's cowering behind a couch while holding a cactus over his head.
The final time we see him before the vault is the sight of him trying to cram his fat body into an air vent to escape you.
FEAR 2: Project Origin
In Project Origin, Jankowski's reactions if you screw around in the plaza.
Griffin: What kept you? Jankowski: Bucket had to take a bath in the fountain.
Everything about Snake Fist.
Stokes: Who the hell are you? Snake Fist: You can call me...Snake Fist. Stokes: (incredulous) ...are you fucking kidding me?!
Snake Fist: You could even say [Alma] covets you. She's a...coveter. You're like free pizza at an anime convention. She can smell you. And she wants to consume you.
Still later, you get to see Snake Fist's little informative video - which he apparently filmed on the toilet using hand-drawn cards. His cartoony naked Alma with big round black button eyes, a frowny face, wiggly hair and a black censor bar across her breasts is particularly silly.
Keegan and Jankowski get sadly little banter, but what is there is hilarious.
Jankowski: A lot of blood. Looks like he got hit in the aorta. Keegan: The aorta? You think it was the carotid or the subclavian artery? Jankowski: Dude, making fun of a man for his knowledge is a sign of insecurity.
A few moments later, after the first encounter with an Armacham goon:
Jankowski: Keegan, you okay? Keegan: I think they hit my aorta! Jankowski: Dude, give it a rest.
Hiding behind the cardboard donkey in Wade Elementary's auditorium causes the enemy soldiers to utter this hilarious line.
Armacham Troops: The ass! He's behind the ass! Shoot the ass!
The linked picture gives a perfect example of the primary source of comedy in these games: the Ragdoll Physics are just amazing, from enemies flinging themselves over guardrails to getting limbs stuck in walls.