The introduction of the Creeper in the first movie. The two siblings are just driving along when suddenly this god-awful horn honks and some Drives Like Crazy dude attempts to run them off the road! Awesome Jump Scare and what a way to introduce someone! This also doubles as terrifying.
The director deserves kudos for some of the film's visual details, like how the state police car's taillights shining behind the siblings' car sends red light straight through the rear and front windows, making the windshield look like it's covered in blood.
Trish repeatedly running the Creeper over with her car to keep him down. It doesn't work, but she deserves points for trying.