Aldo: Donnie! Got us a Nazi here wants to die for his country. Obliiiige him.
Added to that is the utter lack of such "bravery" in their next interrogate, how he gives up everything they want to know, and they keep their word to let him live, setting up the obvious Chekov's Gun of Raine's scarifying his Nazi survivors so he can recognize them "just like that *fingersnap*."
The flashback of when Sgt. Hugo Stiglitz murdered 13 Nazi officers. Major Squick abounds, but either way, it's pure awesome.
Hicox: There's a special rung in Hell for people who waste good scotch. As it seems I might be rapping at the door momentarily... (Downs the glass). I must say... damn good stuff, sir. Now... about this pickle we find ourselves in... it would appear there's only one thing left to do.
Hellstrom: And what would that be...?
Stiglitz (in English, to Hellstrom): Say "Auf Wiedersehn" to your Nazi balls!
When the theater burns as Shosanna's giant face laughs maniacally on-screen, and Donny and Omar shoot the living daylights out of everyone. Bonus points for Donny shooting Hitler's face into pudding.
Shosanna's hijacking the screening, full stop. Unlike the Basterds, she operates with no major plan that can go hare-brained and off the rails, she just edits the film to mockingly undermine the Nazis and declare she has a message for Germany, and then has the place burned to the ground. Even before he's shot to death, Hitler starts freaking out that a Jew has hijacked Goebbels' great work.
The last scene when Lt. Aldo Raine gives Landa a swastika scar on his forehead while Utivitch grins like crazy.
"Nah, I don't think so. More like chewed out. I've been chewed out before."
Doubly awesome for Raine's "The Reason You Suck" Speech right before. He points out that, given the circumstances, he's willing to let Landa have a nice little home on Nantucket Island as a condition for helping the Allies end the war that night. But at the end of the day, Landa is still a Nazi and would try to hide that reality from the rest of the world as he lives in retirement. And, as Raine puts it: "That, I can't abide."
Lt. Aldo Raine: This might just be my masterpiece.
When the Basterds are breaking Stiglitz out of prison, they gun down the Nazi guards, and he just casually sits there without twitching as the guards are being blown away.