After Shosanna shoots Zoller, she looks over at his body while sad dramatic music plays making him seem so very sympathetic. Then subverted as she leans in out of remorse, the whole emotional scene is stopped with Zoller shooting her repeatedly in the gut.
Played completely straight with the farmer at the start of the film. The farmer is probably the most sympathetic character in the entire film. Tries hiding Jews, has a German officer basically tell him "I know the last guys searched up high but I think they're down below and I think they're here" (which they were) so knows that pretending they aren't down there is pointless, knows that his family will be killed if he's found to be lying, and clearly doesn't want to give them up even though he knows there's nothing he can do for them.
When Landa asks Lapadite how many people were in the Dreyfus family, their names, the ages of their children... we already know what's going to happen and it is miserable.
"Oui, Shoshanna." Marcel and Shoshanna have scant screentime together, but their love for one another is palpable even as he goes to die for her.
After Landa leaves Shoshanna in the restaurant, she gives it a couple seconds before completely bursting into tears and hyperventilating. The amount of fear she was feeling through the entire scene comes to a head in that moment, and it's heartbreaking.