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Gothel turning the manor into a tower, erasing the door.
Rapunzel: Why are you doing this? You can't keep me locked away from the world forever. Gothel: Watch me.
Rapunzel and company tricking Gothel into the painting, trapping her in the tower forever.
And using Gothel's own words to do so. Because the tower never releases its prisoner with a "lying heart"—which applies to Gothel herself, not Rapunzel.
Penelope finally standing up to her father, Hugo, and winning his approval—by flat-out ignoring his refusal to hear another word from her and saying, "I'm going to tell you anyway", after which Penelope explains that Rapunzel actually gave up a chance at her own freedom to prevent Gothel from destroying Hugo. And Rapunzel did this because Penelope asked her to.
Tommy firing an arrow at Gothel, saving Stefan. Then the princesses pelt her with food.