- The theme song.
- Gothel turning the manor into a tower, erasing the door.
Rapunzel: Why are you doing this? You can't keep me locked away from the world forever.
Gothel: Watch me.
- Rapunzel and company tricking Gothel into the painting, trapping her in the tower forever.
- And using Gothel's own words to do so. Because the tower never releases its prisoner with a "lying heart"—which applies to Gothel herself, not Rapunzel.
- Penelope finally standing up to her father, Hugo, and winning his approval—by flat-out ignoring his refusal to hear another word from her and saying, "I'm going to tell you anyway", after which Penelope explains that Rapunzel actually gave up a chance at her own freedom to prevent Gothel from destroying Hugo. And Rapunzel did this because Penelope asked her to.
- Tommy firing an arrow at Gothel, saving Stefan. Then the princesses pelt her with food.