Unique Enemies in Donkey Kong:
- Donkey Kong Country has the Rockkrocs in "Stop & Go Station", and the almost completely invincible gray Krushas in "Platform Perils".
- Donkey Kong Country 2: Diddy's Kong Quest has:
- Kackle from "Haunted Hall", another Minecart Madness level.
- The ghostly Mini-Neckies that show up during the Kreepy Krow boss battle.
- Snapjaw appears in two levels, and is the only instance of such enemy in both.
- Many examples in Donkey Kong Country 3: Dixie Kong's Double Trouble!, often serving as a level's gimmick (However, most of these enemies are seen more than once in the Game Boy game Donkey Kong Land III, which is based off of DKC3):
- Bazza the barracuda shows up in only one of the game's many underwater levels, serving more as barricades to bypass than enemies. The Game Boy Advance remake includes them in some bonus levels, however.
- The bird enemy Swoopy serves a similar purpose in one of the game's later levels, Swoopy Salvo. However, you can find just one other Swoopy in one of the earlier tree-themed levels.
- Lemguins appear only in the last level of K3, popping out of holes and sliding towards you.
- Karbines serve as indestructible enemies in the first factory level, hiding in the background while shooting fireballs at you. Similarly, Kroc from Krack Shot Kroc does this, except from the fourth-wall side of the screen. Although you play as Kroc in one of that level's bonus stages.
- Kuchuka is a purple barrel that chucks bombs at you, appearing only in the level Pot Hole Panic.
- Pink versions of the enemy Koin known as Koindozers show up in one of the levels in the last world, attempting to push you off ledges with their trash-can lid shields.
- Minkeys—little monkeys that chuck acorns at you from behind barrel-shields—are only seen in one of the second world's levels, though they made an additional appearance in one of the remake's extra levels.
- Gleamin' Bream, whose only purpose is to light up dark areas, in "Floodlit Fish".
- Finally, the remake adds in a ground-based version of the TNT barrel Klasp to one of the new levels, named Kracka which acts similarly to the Knocka enemy.
- Donkey Kong 64:
- The Killer Tomatoes. They only appear in one specific area of Fungi Forest, and after you kill them, they're gone for good.
- There's also the unnamed, clam-like monsters that are found in a treasure chest only Tiny can enter in Gloomy Galleon.
- The bonus games have some examples, like the Red Kritters from Kremling Kosh and the burly Kops from Stealthy Snoop. Those do not exist in the main game.
- The battle against the Toy Monster from Frantic Factory includes Chunky fighting off some regular toy enemies beforehand, like the walking dices and domino pieces you find in the Testing Room. However, you also have to fight off a set of living rulers that are found nowhere else in the game. And there's a block enemy that you can't fight at all, only existing to help form the Toy Monster.
- The Giant Spider boss of Fungi Forest can summon its offspring to attack Tiny Kong. Suffice to say you can't find those anymore once you defeat their mother.
- Living flames wearing sunglasses appear in one of Crystal Caves' igloo puzzles, but only there.
- Creepy Castle's library has some evil living books that are exclusively found there.
- Klobber, a common enemy in Donkey Kong Country 2, appears in only one part of one level in DK: Jungle Climber.
- Donkey Kong Country Returns:
- Kowalees are purple koalas wearing neckties who throw bombs at you. You only encounter them in the very last level, Golden Temple, and this is also their only appearance in the entire franchise to date.
- Button Bash of the Ruins world features a room containing 6 Humzees. These hummingbird like creatures try to charge into you with their sharp beaks and defeating them are required to proceed through the stage.
- Also from the Ruins world is the Giga Rangwi a King Mook of the shark-like Snaggle. This giant piscine is only found at the very end of Damp Dungeon, right before the goal. Since the specific stage it is found in is optional, it means that it's possible to miss it on an initial playthrough.
- Both the standard and MK II Pyrobots appear multiple times in the Factory world so they don't count for this trope. In the Updated Re-Release for the 3DS though there is a variant of the standard Pyrobot that shoots fire upwards instead of to the side which only appears the version exclusive Robo Factory stage.
