- Actor Allusion: Skylar's daughter asks her at one point "Are you a terminator?" She is.
- California Doubling: Set in New York City, filmed in Toronto.
- Fake American: Ryan Cartwright (Gary) is actually from England.
- Filming Location Cameo: At the end of the season 1 episode "Catch and Release", some of the characters travel to Toronto.
- Follow the Leader: It has some similarities to The 4400.
- Playing Against Type: Skylar's first episode initially appears to portray her as the kind of crazy superpowered teenager that Summer Glau was famous for playing in Firefly and Terminator: The Sarah Connor Chronicles. This makes the revelation that she's trying to save her own daughter much more of a surprise, since nobody would expect that type of character Glau normally played to be old enough or stable enough to be bringing up a kid.
- Screwed by the Network: It took around three months for Syfy to announce the show would be cancelled, after so much hope was generated for season three. An interesting example because Ryan Cartwright (Gary's actor) initially posted on his Twitter that he will assume Alphas is cancelled after waiting so long to "decide" and this forced Syfy to react and contradict him. Of course in the end, it was still cancelled, serving as a Kick the Dog moment for the fans.
- You Look Familiar: Brent Spiner has appeared in both Alphas and Warehouse 13 playing different characters. Notable as this is the first time they've reused an actor in a different role since the series was established to exist in the same universe as Eureka and Warehouse 13. note
- Kimi Milard (Sarah Slywchuk) actually had one openly spoken line of dialogue in the entire second season, though it was cut for unknown reasons.