Please don't list this on a work's page as a trope. Examples can go on the work's YMMV tab.
Tear Jerker: Nausicaä of the Valley of the Wind
The treatment of the baby Ohmu in Nausicaa of the Valley of the Wind gets this devout insect-lover wibbling like crazy. The humans capture it, spear it all over, and drag it through the air, squealing pathetically and bleeding, as bait to draw out the parents, because Humans Are Bastards. Sure, everything turns out okay in the end, but the moment is definitely there.
When Nausicaa tries to keep the baby Ohm from moving and her foot its the acid lake ... lets just say that it is hard to forget her heroic pleading screams. Pass the tissue please.
Teto's death in the manga.
Also in the manga, Lord Yupa sacrificing himself to protect Kushana. He smothers a grenade in his cloak, and even though he doesn't flinch when it blows his arm off, at that moment you realize he's a goner.
