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Tear Jerker: Lone Wolf and Cub
Ittou's speech to Daigorou shortly before the final battle in Lone Wolf and Cub. ("Though my eyes shut, my lips close... do not fear. In that world of rebirth, I'm still your father. In that world and all worlds, *you* are my *son* !") Daigorou remembering this speech at the end of the battle, when realising his father is dead, is even worse.