The end of theKarin The Chibi Vampire manga. Not only does Karin's older brother Ren wipe all her memories of her family but he does it with his last and only sign of brotherly affection. A pat on the head, because 'you don't like it when I'm rough, do you?' And we learn that all those times he gave her noogies were to help the memory wiping process and Dear God, her family was planning this from the start because she wasn't a normal vampire and they wanted her to have a normal life in the end. Which they could never give her. And then, on top of that, we get the most bittersweet moment in the manga when Usui Kenta asks Karin if she remembers her family and she replies 'I don't have a family' while crying without knowing why, making Kenta cry as well- and then, he finally proposes making it so so wonderful and painful at the same time.
Anju awakening as a vampire, leaving her life with Karin behind. The poor girl breaks down into tears after hearing an Innocently Insensitive "congratulations" from Karin, and she cries for the rest of the night.