En route to the hospital, plants take Pamela Isley and transform her into Poison Ivy. Ivy proceeds to use her new powers to attack ChloroGene, and then GCPD. Barbara puts on the cowl for the first time as Batgirl to fight her former friend, and earn Batman's respect.
This episode provides examples of:
- Blind Without 'Em: The first time Gordon sees Batgirl is after Ivy destroys his glasses with plant acid.
- Book Ends: This episode ends with the same line Barbara (via voiceover) said at the Cliffhanger ending of part 1.Barbara: Because this, my friends, is only the beginning.
- Doubles as a Meaningful Echo, as before it was signifying trouble as Pamela was beginning her transformation as Poison Ivy, while here it’s signifying the start of Barbara’s new life as Batgirl.
- Born from Plants: More like reborn from plants but the birth symbolism is front and center as Pamela Isley becomes Poison Ivy.
- Bound and Gagged: Ivy gags Gordon with a plant vine when he warns Batman that he's walking into a trap.
- Brainwashed and Crazy: Ivy finds out her breath can cause this to happen. She uses it successfully on Batman.
- Buffy Speak: Batgirl after Batman calls her Barbara complains that he broke the "secret identity rule".
- Deducing the Secret Identity: Barbara to her credit makes a "Batwoman" costume that covers her eyes and most of her hair, so that her dad doesn't recognize her without his glasses. Bruce, however, figures out immediately that "Batgirl" is Barbara Gordon for a good number of reasons: her hair color and voice are the same, and they move with a gymnastics style while fighting. Plus, Barbara said she wanted to help Bruce save Pamela, and he rebuffed her.
- Don't Tell Mama: Batman gets Barbara to enter a 10-Minute Retirement by threatening to tell her father, and plans to do so anyway. He goes back on it when she helps him take down Poison Ivy and save her father.
- Fighting Your Friend: Barbara is forced to do this to save her father and stop Ivy. She has a sad look by the end of the fight.
- Garden Garment: Pamela's metamorphosis pod that turns her into Poison Ivy dissolves her civilian clothes but conveniently, Ivy is able to grow leaves from her body shaped into a dress
- Good Old Fisticuffs: This is how Batgirl breaks Ivy's mind control on Batman.
- Glowing Eyes of Doom: Pamela sports them before and during her transition to Poison Ivy.
- How We Got Here: As the episode catches up with the previous one’s Batman Cold Open, Barbara skips over it, beyond the specific details, before the plot resumes.
- "I Know You're in There Somewhere" Fight:
- Averted when Ivy visits Barbara and offers We Can Rule Together. Barbara still calls her Pamela and points out that she's a "plant". Pamela isn't so far gone that she ignores this.
- Batman tries this on Ivy, saying she isn't well. When Batgirl tries the same thing, Batman mentions that it doesn't work.
- Commissioner Gordon pulls a gun on Ivy when she attacks his men and tells her they don't have to fight. Ivy says it does and kidnaps him.
- Insistent Terminology: It's not Batgirl, it's Batwoman. By the end of the episode, Barbara accepts the Batgirl moniker.
- Jumped Off The Slippery Slope: Ivy goes from taking down Chlorogene vigilante-style to kidnapping and preparing to kill her best friend's father.
- The Load: Averted with Barbara. She delivers crucial evidence to Batman after using the Bat signal, saves him from a rogue plant that attacked him from behind, and breaks the brainwashing on him. She also saves her father and delivers the final blow to incapacitate Ivy.
- Morality Pet: Barbara's the only human that Ivy treats with kindness, offering her We Can Rule Together and a flower, and giving her time to think about it. Although this doesn't stop her from attempting her kill her friend's father.
- Mythology Gag: After hearing about Batgirl, Alfred snarks that maybe he should build a doggie door for Bat-Hound.
- Oh, Crap!: Batgirl gets unnerved after Batman casually deduced her identity. She quickly tries to deny it, but he wasn't fooled in the slightest.
- Paper-Thin Disguise: Averted; Barbara's costume covers her eyes and most of her hair. The thing is that Batman recognizes her voice and form from her using the Bat signal, and he deduces her identity quickly.
- Pet the Dog: Even after her transformation, Poison Ivy still treats Barbara like a good friend when she goes to see her before beginning her attack on Gotham.
- Reasonable Authority Figure: Batman overall acts this way towards Barbara. When she turns on the Bat-Signal, he tells her that it's not a toy but he becomes serious when Barbara says that Pamela showed up in her room all hopped up on Chlorogene chemicals, taking the sample flower that she provides him. Batman refuses to let Barbara come with him to stop Poison Ivy because it's dangerous but promises her in Sincerity Mode that he'll do all he can to save Pamela from herself. When she proves herself incapacitating Poison Ivy, snapping Batman out of a brainwashed spell, and rescuing her father, Batman says that he won't tell her father for now.
- Rule of Three: When Batgirl throws things, she only manages to hit her target the third time— using a homemade Batarang to save her father from Ivy's plant monster.
- Something Only They Would Say: Barbara at first doesn’t recognize Pamela after her transformation into Poison Ivy, until Ivy calls her “Red”.
