Quotes / Viewer Gender Confusion

Hobbes: If we get in National Geographic, maybe I'll get to meet some of those tigress babes they showed in the April issue! Yow wow!
Calvin: Those were females? Really, I don't know how you can even tell the difference.

Krillin: These aliens are scary, especially that one in the front. Looks like a total FAG.
Krillin: What? A Freaky Alien Genotype. What'd you think I meant?
Gohan: Oh, I thought you were calling him a derogatory term for a homosexual.
Krillin: THAT THING'S A GUY?!?!?

"Cripes! I thought that was a girl!"
ShogunGin0, reacting to Thistle removing his hat and beginning to speak in The Elm-Chanted Forest.

...wait a minute...
* Beat*
* Beat*
...Stop it, boner.
Rainbow MotherFuckin' Dash ‎, [eponymous blog]

Meinos: Upon intrigue, the White Overlord tells Takatora that- {Record Needle Scratch}
Roshuo (via subtitles): It seems she (the she in question being Redyue) wants to apply human technology in an attempt to resurrect my Queen.
Meinos: She? The... Green Bishop... is a she?! With THAT voice?! I-ih-hoh... How was I supposed to... DAMN YOU GAIM! You're screwing up my Chess Motif! Who's heard of a female bishop?!
Meinos Kaen upon the revelation of Redyue's gender.