Sadly averted in the original 300 graphic novel, and former Trope Namer. No emphasis was placed on this sentence when Leonidas kicks the messenger into the well. Just, "This is Sparta."
In fact, there seemed to be a lack of "passion" by all the characters in general. It was almost as if they were going through a routine they had grown weary of.
In Alan Moore's Supreme, the unseen villain The End says everything like this, each word being afforded its own speech bubble. For example, when addressing some lesser rogues gallery members: "YOU." "ARE." "SPECKS." "GO." "AWAY." They promptly fled the room.
Justified in The Long Halloween, as Catwoman is currently trying to kick the ever-living crap out of Batman for foiling her first attempted robbery of the Maroni family:
In #124 of The Avengers, Black Panther does one to an incarcerated supervillain when he needs instructions on how to use his weapon:
Black Panther: Van Lunt, I swear to you that lives of everyone on this planet are at stake! We — Need — This — Weapon! Van Lunt: Well—, when you put it like that—! I guess... I won't be needing it anyway.
The DCU supervillain Dr. Light delivers one in Identity Crisis when he realizes the full extent of the Cerebus Retcon.
Peter Parker: I'd. Like. To. Have. A. Cup. Of. Coffee. Right. Now.
In one issue of Batman: The Brave and the Bold, he gets a distress call from General Immortus. When he gets there, he finds Ancient Greek troops, with red capes, spears, and everything, have captured the General. They attack him.
Batman: THIS-IS-NOT-SPARTAAA! (chestkick)
The Flash villain the Turtle speaks like this. "I... heard... about... his... mother... in... the tabloids... Golden..." "Glider, Golden Glider! Cripes, old man, talk!"
In Mighty Avengers #23, when Iron Man shows up to lead the new Mighty Avengers at Wundagore (which is being besieged by Chthon in Quicksilver's body), an upset Hank Pym (now the Wasp) demands a good reason to allow Tony Stark ("the man who fought Captain America in the Civil War. Who sent the Hulk to outer space and pitted him against the world. Who gave the Skrulls all they needed to invade the Earth") take over. His response: