Especially memorable as the three characters who are not Doris are all ANGELS. The one who explicitly admits to being scared of her, and that Doris has far worse in store for the girl (Lucifer's niece), is an Angel of Vengeance.
In A Very Potter Musical, Molly Weasley and Rumbleroar are each in only one scene, but no one is likely to forget either of them.
In Suburban Knights, a girl using a cellphone who the villain stumbles upon and kills got quite some attention from the fandom, ever since her one second appearance in the trailer. The director/star even discusses her on the DVD Commentary (she is played by an ex-girlfriend of his).
Sonic the Hedgehog 2: Special Edition. Failure Cresh, one of the series' few original characters, appears in only one level for about five minutes (and later gets a brief mention in the epilogue). In this short time, we learn his entire (hilariously) tragic past and see him (mercifully) die. Consequently, he makes a big impression on audiences.