- The sole concept of being the last human alive on Earth. At least in the latest film adaptation, Neville is aware that there were few millions of people who are naturally immune all around the world and there is a good chance some of them are still alive. Maybe not around New York, but someone somewhere could survive, giving a sliver of hope. Not so much in the book, where he doesn't even know what is happening early on.
- Your own, beloved wife coming back from her grave. A grave you've put her in yourself. And yearning for your blood. And still being apparently at least semi-sentient, so it's not just a mindless, blood-thirsty undead you can rationalise into killing in self-defense.
- Neville going into a darkened building, a likely place to find lots of Infected, to search for his dog. He's so obviously terrified you can't help but be frightened with him.
- The zombies look very frightening.
- Some of the Poster art made for the film is pretty creepy, ranging from the Quarantine Public Service flyers to one specific propaganda poster in the opening scene, with a picture of an infected individuals Bleeding face, save for the eyes covered up by a cutout Michelangelo's The Creation Of Adam, specifically the image of God and Adams Index fingers touching, with Bold text God Still Loves Us, while at the bottom of the poster, there is an illustrated parody of the Michelangelo cut out, depicting a human hand holding a gun at God, with a crudely inscribed line Do we still love God?.
- One can't help but feel unnerved when Neville finds an immobile mannequin standing in the middle of the street, far from where it was supposed to have been resting.
- The news reporter describing the ongoing mutations being caused in those treated with the Krippen virus, showing how something that should have been a source of hope has gone so badly wrong.
Reporter: So far, almost 5000 patients treated with the retrofitted virus have begun exhibiting symptoms resembling the early onset of rabies. Twenty five patients have already died.
- The evacuation scene, and seeing the panic as hundreds of people are trying to flee New York.
- A moment that really stands out during this segment is Neville, while waiting with his family to get through the military checkpoint out of Manhattan seeing a woman insisting she is uninfected and pleading with anyone taking to take her child with them out of New York. As Robert watches, he sees the woman start bleeding from her eyes and his saddened expression as he knows that there is nothing he can do to help her is chilling.