I Am Not Your Father
The Reveal that his parents are not his real parents after years of Oblivious Adoption. This is when after a long time, the adoptive parents finally tell him. Don't confuse with I Have No Son or That Thing Is Not My Child!. Also, this is not simply a subversion of Luke, I Am Your Father; this trope only applies if the non-father was actually believed to be the father.
- In more than one incarnation of Superman's origin story, young Clark Kent is blissfully unaware that he's an alien from the planet Krypton until he comes of age and Martha and Jonathan show him the space ship.
- In Violine, Francois reveals that Marushka, who claimed to be her mother, was actually his governess, and her REAL mother is missing in Zongo.
- This◊ Bad Habits strip.
- Half an example occurs in the fanfic The New Retcons, where Michael Patterson discovers that John was not his biological father, and had in fact adopted him when he married Elly
- In Kung Fu Panda 2, Mr. Ping finally admits that he's not Po's actual father. Of course, since Po is a panda and Mr. Ping is a goose, it was obvious to everyone else, but they just didn't talk about it.
- The trope was subverted in the original, however. Mr. Ping tells Po the secret ingredient to his soup instead.
- In Disney's Hercules, after Hercules ends up smashing up a lot of stuff in town because he can't control his strength, his mortal father decides to tell him that he's adopted and gives him the medallion with the symbol of Zeus on it, which leads him to the temple.
- Monty Pythons Life Of Brian:
Brian, Mr. Cohen wasn't your real father.I never thought he was, Mum.
- The protagonist has a moment like this at the end of Shades of Grey (though it's the result of illegitimacy, not adoption).
- In the Star Trek Expanded Universe novel A Stitch in Time, Garak remembers coming home to his dying father's side. Over his mother's objections, his father reveals that he's actually his maternal uncle, paid by Enabran Tain, Garak's real father and the head of the Obsidian Order, to maintain the ruse. Interestingly, the episode of Star Trek: Deep Space Nine that first reveals this to the audience has Tain denying the claim in this manner at first until Garak begs him to speak the truth for once in his life.
- Supernatural, episode 4x10, "Heaven and Hell." Anna Milton discovers that her parents are not her real parents, and she is in fact a fallen angel that was reborn as a human.
- In the Syfy mini-series Tinman, DG's parents revealed to her that she's adopted and that they're actually robots.
- Parallax: Jeremy is not Ben's father. Veronica and Jeremy already knew this, but the rest of Ben's blood relations didn't. Of course, the truth was just too damn weird for anyone not Veronica to accept.
- In the Syfy series Haven, Chief Wuornos tells Nathan "I'm not your father" after Nathan confronts him about his Trouble being the same as Max Hansen's.
- Subverted in Tales of Symphonia. It's likely that Frank actually is Colette's biological father; Remiel lied when telling Colette that he was her true father.
- Dragon Quest IV, at the beginning of the Hero's chapter.
- Dragon Quest V: None of the Heavenly Brides have real parents, and are actually descendants of Zenithians.
- Super Mario RPG: Frogfucius dramatically reveals to Mallow: "You... are not a frog!" (Since Mallow is a walking cloud creature, the audience isn't surprised.)
- Everyone else at Tadpole Pond is shocked. Complete with scare chords accompanying the reveal.
- ''Gun' has the main character find out that the man who's raised him since infancy and trained him in hunting and gunfighting isnt his father at all, moments before said man is killed in an outlaw attack. The early parts of the game revolves around figuring out who his real parents were. They were a white frontier doctor and an Indian woman, who were murdered by the Big Bad for their knowledge of an enormous treasure. The man who raised him had in fact been working for the Big Bad as a guide, only to be betrayed when he attempted to stop the massacre.