The beginning of the first movie, when the two brothers hug after Jake is released from prison. Doubles as a Establishing Character Moment, since neither of them had said a word yet.
Elwood reluctantly letting Jake sleep in his bed, taking the chair for himself.
He decides to stop trying to wake Jake up and instead decides to place the only available blanket on him. He uses his jacket instead. Brothers indeed.
The "Stand By Your Man" sequence.
Except for that one trucker. Poor guy.
The performance of "Everybody Needs Somebody" will make you feel good in so many ways.
Elwood: People, when you do find that special somebody, you gotta hold that man, hold that woman! Love him, please him, squeeze her, please her! Signify your feelings with every gentle caress, because it's so important to have that special somebody to hold, to kiss, to miss, to squeeze, and please!
Cabel performing "John the Revelator" with Reverend Cleophus James and Sam Moore, proving himself just as good a showman as his father, and taking his true place with the band.
Buster talking Elwood into continuing on ("the music, man, you know you'd miss the music"), and Elwood's Rousing Speech to the band about why it's their duty to finish what they set out to do.
The fact the film is about the two of them trying to save the orphanage with the band.
Elwood taking a moment of reflection after the faithful, battle-hardened Bluesmobile completely falls apart, dying the very second its role in the drama had ended...
The "Shake Your Tail Feather" sequence with Ray Charles, both in the movie and in a meta way. It's very clear that John Belushi and Dan Aykroyd are having an absolute blast dancing together and therefore, so are Jake and Elwood.