One particular favorite came in the very first episode. The child actress playing the lost girl the heroes are meant to be helping makes a very visible WTF Face as Major Victory insists upon carrying her, Superman style, to the nearby police station.
After the Crowning Moment of Heartwarming of him receiving a call from his estranged daughter, he looks up at Stan and jokingly asks if he can keep the phone that Stan provided for the call. Stan has a good laugh at that one.
"I know Major Victory can't keep his costume on. But Thong Man, now we haven't been introduced to that particular superhero."
Major Victory tells Dark Enforcer that he's going down for this. Dark Enforcer responds by waving a dollar in front of the screen.
In episode 2, during the costume update, Stan tells Lumeria that,, with regards to her costume, "Disco is from the 70s". Her response is to go "Hahahahahahahaha-So am I."
Feedback's failure to impress the kids. At one point he says his favorite video game is pong, and one little girl says, in a talking head interview: "I've never heard of the game Pong. I think he made it up."
And in the second season:
Ms. Limelight, who is The Ditz to hilarious levels.
Mr. Mitzvah, during both challenges and eliminations.
Hyperstrike, a young and energetic Large Ham with acrobatic skills.