Commander Up's story about his injury and why he's such a softie has to be seen to be believed. The fact that it's Joe Walker crying onto Joey Richter's shoulder and sitting on his lap the entire time makes it that much funnier.
The end of that scene where they nuzzle their noses together just put this troper in hysterics.
And the whole "Christmas Shoes" part was just brilliant.
"Hotdog style. Not hamburger."
—>Up: I've never really told this to anyone before.
—>Bug: Well you've never been on someone's lap before now either!
During "Beauty", Bug starts getting into the song (because he can understand what all the bugs are singing) while February just stares at him with a "WTF?" expression on her face.
The whole scene between Pincer and Junior.
I... am not a dumbass. He's a dumbass!
February and Bug's kiss. Joey Richter's Aside Glance to the audience is just icing on the cake.
You are the bravest sonuvabitch I've ever met in my life!
The blue grasshopper-esque bug trying to make Bug think planting eggs into mammals seem like the greatest job in the world:
Egg-planter bug: "So, ya still think being an egg planter is lame?"
Bug: (after a moment's pause) "...yes."
For that matter, Nick Lang's blue over-enthusiastic instructor bug in every scene he's in.
Why don't you just got ahead and devour the nearest mammal!
After Bug is sentenced to be an egg-planter forever and never leave the Hive, the Mosquito Brothers come by and try to mock him. Bug then says something along the lines of "Oh, sure, kick me while I'm down, why don't you" and they gladly try to slap him (with their own grunting sound effects) while he just stands there. While this is going on, Joey casually scratches the back of his neck. When the mosquitos see it isn't working, one of them says "What the hell?"
This little exchange.
Commander Up: You just stared down a gigantic spider, and in my book that makes you one tough son-of-a-bitch.
Bug: Thank you sir! (raises his right arm into a salute) I am a tough bitch!
The whole bit where Roach gets selected as a Queen Impregnator.
Commander Up's speech about how he pee pees like a big boy
Commander Up: I do not pee pee sitting down! I never did it and the one time that maybe you heard about it was because the gravity was on too high. So you try peeing while the gravity is THREE times too high! Alright! I don't pee pee sitting down! I pee pee like a big boy, dead God dammit. I pee pee like a big boy so stop making fun of me!
"All hail Astro Boy!"
Junior's reaction to Bugette's larvae.
Are... Are they friendly?
Junior getting choked.
"Step off skank. You do not know me."
Taz. Just Taz. All her lines are hilarious.
Taz:Put your hand on that wall.
Krayonder: What? Why?
Taz: Put your hand. On that WALL!!!
Taz: You'd better unfudge yourself or Commander Up will unscrew your head and drop the wishing pennies down your throat.
"Now take a walk off my knife!"
And who could forget:
Taz: I will wrap you up in a tortilla, and EAT YOU. As a snack.
Taz: If you don't go out there and die for something... I will KILL YOU FOR NOTHING!!
Taz: "Oh, don't throw the knife? OK."
Taz: "Oh, does that hurt you? WALK IT OFF! *repeatedly stabs herself with knife*
Taz: "Get your zappers and set them to pew. Pew pew, pew pew!"
Every reference to Dead God.
That moment when, after Up has killed the Mosquito Brothers, the puppeteers leave their puppets on the stage floor and just casually walk off midscene.
After watching the captain's log of a failed expedition to Bug World, which culminates in the captain being cut in half by Pincer, Bug nods and says "That was a good video. Until the end, when it got sad." It really must be seen to be believed.
Part this, part Tear Jerker. When the Overqueen appoints a certain bug to keep the peace: