Anytime Kenny gets the Level Three Aku Aku mask, Trevor sings a version of the invincible song.
Getting 10 extra lives by bouncing on a poor polar bear's head. That poor bear.
Apparently, Cortex's massive chin is bigger than Batman's.
Kenny intentionally belly flops onto a pile of Nitro Crates during a Bonus Room. In fact, the fail was so epic he showed it three times, the third time slowed down with the Leeroy Jenkins sound byte.
Any instance of the time cards.
There's a part near the end of the game where Kenny kept unintentionally running into a Nitro crate or an enemy, or sometimes being pushed into a Nitro crate by an enemy. He only shows it four times, each time intercut with a time card, but by the end, he had gone through seven lives before finishing. Still, it was friggin' hilarious.
Kenny goes on almost non-stop about how much he hates the puzzle at the end of the game, so much so that he needs a certain clip from Mighty Morphin' Power Rangers just to show his frustration.
Rita Repulsa: Oh, I've got such a headache! Somebody bring me an aspirin!
One ugly motherfucker.
At the end of Part 3, Kenny saves in a small storage room just after meeting Carlos. At the beginning of Part 4, he gets ambushed by Nemesis upon leaving the room and dies. All the while he's just freaking out.
Jesus Christ! Jesus Christ! No! No! Nonononono!
And apparently, he was so flabbergasted he had to see that scene twice.
What just happened?
Imma firin' ma laser!
Kenny re-enacting the "Planning To Fail" PSA from Red VS Blue. (This was actually during one of the failed attempts to record the game, but it still got a laugh from Trevor, so it counts)
There are three instances of the WTF Boom. The first two involve Nemesis getting blown up. The first time this happens, in the restaurant, he turns in time to the "WTF" before the "Boom". The second time is during Mikhail's Heroic Sacrifice, and the third time is when Nemesis blows up the rescue helicopter.
ZUUL, MOTHERFUCKER, ZUUL!
When Kenny pushes Nemesis off the clock tower balcony, he edits in the Goofy Yell. He does this again when he pushes Nemesis off the bridge between the park and the water treatment plant.
When Kenny encounters Nemesis in a small hallway after completing the scavenger hunt, he puts in a picture of a cartoon mouse screaming "I want my mommy!"
While in the sales office in Part 8, after watching the TV to get the password to unlock a door, we get a bit from Spaceballs where the movie (or game, in this case) is turned off partway through.
Also while in the sales office: As the zombies come bursting in through the window, we get a sound byte of the Kool-Aid man saying "Oh yeah".
SNAP INTO A SLIM JIM! OH YEAH! (followed by Benny Hill)
Saria and Zelda using their ocarinas as vibrators.
Part 14 has the Trololo song playing as Ganon is going on about how Link actually helped him get into the Sacred Realm. At the end of the cutscene, as Ganon laughs, it looks like he's actually singing along with the song.
Before that, Trevor gets trolled by the bomb pot in Goron City.
Alanie gets drunk.
Anytime there's a jump cut, Trevor edits in "PINGAS!"
Trevor's idiot moment in the Water Temple, causing Porky Pig to hijack the commentary
Whatever Alanie is doing with that goat, we don't want to know
Part 23 is nothing but Alanie forcing Kenny to bleach his brain with her dirty mind.
Before this, Kenny kept trying to shoot the owl, only to end up missing. The joke is that he was drunk, even though he'd only had one beer and a cup of soda.
Trevor totally annihilates the Water Temple boss.
All throughout the Water Temple, Kenny — using the voice of Nappa from Dragon Ball Z Abridged — kept asking "Are we done yet?" prompting annoyed groans from Trevor and Chris. Trevor kept telling Kenny to shut up, but he kept doing it anyway, even AFTER the Water Temple.
Kenny: Are we done yet?
Trevor: Shut UP! We're done already!
While trekking through the child half of the Spirit Temple, Trevor traps a floor spike between a block and a wall, causing it to jitter back and forth; the guys all say that that's how they got their name Spaz Attack.
And later on, the floor spike clips through the block, damaging Trevor.
In Part 1, Anthony makes note that the Morph Ball is one of his favorite Metroid power-ups, not realizing that he had just set himself up for a joke about how he likes balls.
BSL = Bullshit Lounge.
Trevor decides to try to take on the SA-X, but it goes about as well as you would imagine. He survives by running away, and as he runs, he edits in a scream by PewDiePie.
Samus vs. the Mexican Jumping Condom.
When Trevor's recording craps out, he leaves a message saying that they'll be using Kenny's Skype recording, accompanied with the image of said Mexican Jumping Condom wearing a sombrero and ranchero music playing.
Guile's Theme goes with everything.
Way back in Part 1 when Trevor stopped at a navigation room because he forgot what he was doing, Kenny called Samus an idiot. Said remark got the ''Big SHUTUP" from Samus, as well as a picture that made it look like she was about to shoot Kenny in the face.