Guido's pit stop is without a doubt the Crowning Moment of Awesome for this film. The reaction of Chick Hicks' pit crew is priceless - they drop their mustache grilles. "Peet stop."
Lightning muttering to himself about his failed use of Doc's advice.
Lightning: "Turn right to go left"...Guess what? I tried it, and you know what? This crazy thing happened - I went right! Lizzie: You keep talkin' to yourself, people will think you're crazy. Lightning: (sarcastically) Thanks for the tip. Lizzie: What? I wasn't talking to you.
Harv buttering up Lightning:
Harv: And it is such an honor to be your agent that it almost hurts me to take ten percent of your winnings. And merchandising. And ancillary rights in perpetuity— anyway, what a race, huh, champ?
And later after Harv finds Lightning:
Harv: "You can't buy this kind of publicity! I don't know what you need me for! That's just an expression, by the way, you signed a contract."
What really sells this moment is his change of tone, mid-sentence. At first it's loud and hammy, then it's all serious and quiet, with no warning.
"AND MY NAME'S NOT CHUCK!" Even better is that his character is listed in the credits as "Not Chuck".
Comes up again in the mini-biography collection you can unlock on the Blu-ray. Actually most of the biographies are pretty funny.
Mater: I know this might be a bad time right now, but you owe me fifty two thousand dollars in legal fees.
This exchange between Fillmore and Sarge:
Fillmore: "I'm telling you, man, every third blink is slower."
Lightning's Freak Out when Van and Minny quickly flee Radiator Springs, while he's stuck to Bessie, flailing around:
Lightning: Don't leave me here! I'm in Hillbilly Hell! My IQ is dropping by the second!! I'M BECOMING ONE OF THEEEEEEEEEMMMMMMMMMM!!!!!!!!!!!
The very final scene after the credits, where Van and Minny are lost in the desert.
Really, Mater provides pretty much all the Funny in this film.
Mater gets some drinks for Lightning at the free bar in Japan. Those who tried their first "pistachio ice cream" knows what's coming a mile away.
Sushi vender: My condolences.
The scene where Mater actually freaks out after seeing a car with headlights for eyes in Paris.
What made this scene is that this is seemingly a reference to the decision to avoid that sort of character design during development of the original Cars as it made characters look like "snakes".
He tries to crash Lightning's and Sally's date at the Wheel Well by posing as a waiter. He even tries a drink at one point, hates the taste, nonchalantly sips it back out into the cup, then almost serves it to Lightning!
While tearing through Radiator Springs on his new jet engines, he comes across the car he towed in the beginning of the movie. He tows him to the shop, hooking him so fast he pulls him right out from under the dust he'd accumulated.