Achmed Khan's story about a rock concert at his school. When he gets to the part where one of the guitarists broke a string, he lets out an unexpected crazy scream.
Dante Robinson's chatter, especially "I see London, I see France, I see your underpants!"
Keisha Phillips has a couple of good lines.
Keisha: (during the chatter) Watch out, Batman! Here comes The Joker!
Keisha: (when getting picked) I knew you were smart, a real stinker—I mean thinker.
One of the phrases Pablo Sanchez says when he strikes out is "Este bate no sirve para nada." which translates to "This bat is useless."
During the credits:
No hot dogs were consumed in the making of this game.
Vinnie: (when a generic kid steps up to bat) I wouldn't want to meet this kid in a dark alley. I wouldn't want to meet him in a light alley, either. Alleys are scary!
Vinnie: (when two or more fielders collide) Pig pile! Everybody jump on!
The strikeout animations are also pretty funny to watch as well. Complete with wacky sound effects at that!
When Pete Wheeler strikes out he drops his bat and them clumsily steps on top of it while a crazy drum kit plays away during his tumbling.
When Ricky Johnson strikes out, he looks at the camera with a disappointed look on his face, then he backs away step by step slowly before just... flinging his entire body off screen.
Backyard Soccer
Angela Delvecchio recounts a story where her family paid a visit to Vinnie the Gooch's house. Vinnie told a joke that made Tony laugh so hard that milk came out of his nose.
Backyard Football
Achmed Khan's "rock song".
Achmed: Rock! Rock! Everybody rock! Everybody love the heavy rocking guitar! Yeah!
One of the rejected names for Achmed Khan's band was Ham Sandwich.
If you hold down Shift while clicking on Pablo Sanchez, he will say in English that he dreamed about a burrito.
Pete Wheeler's dad apparently holds the record for most rushing yards in a single game of Backyard Football. Only for all those yards to be going the wrong way, resulting in multiple safeties for the other team. The other team won.
When Dante Robinson scores a touchdown, his celebration is running to the concession stand and running back with a tray of food and starts eating it in the endzone.
In most of the later games, when you create a custom player and don't give him any stats, they tend to just use all the "(s)he's not the best in [category]" type description for every stat. In this game, however, if you make a custom player and leave all the stats at one, the description? "This kid is not a strong player."
The player bios have two ways of explaining how Dmitri Petrovich got his nickname of "Paste". Dmitri will be upfront about it but Jorge Garcia's depiction is funnier.
Jorge: You haven't by chance seen Mr. Petrovich around, have you? He's the rather large boy who eats paste.
Backyard Basketball
In the original game, Barry DeJay would have an interview with the 110% Juice Player of the Game at the end of the game. Usually the question is straightforward and relates to the game, but sometimes Barry will ask an off-the-wall question, such as whether he should get a mustache or whether Iago from Othello was a tragic figure. The Player of the Game will comment something to the effect of either "I have no idea what you said" or "You're crazy".
Custom player: I just gave 110... what did you just say?
Special mention goes to Lisa Crocket, who just doesn't respond at all.
Backyard Hockey
Sunny Day poking fun at Buddy Cheque for wearing a girl's jacket, with the latter getting defensive. Turns out, it's a hand-me down since Buddy has six older sisters.
Buddy Cheque ending a game commentary with a word from a sponsor.