The Overture March (also known as the Dragon Quest Medley or just Overture). (Symphonic.) Behold, a song instantly recognizable instantly to the entire country of Japan for the past 25 years, it has been remixed literally thousands of times and prefaces every Dragon Quest game.
The Overture remix, that only plays after we see the Hero prepare and proceed on his journey or rather, that we see that he has failed on his journey, or more specifically (in the GBC remake) when the king declares that you are the inheritor of his legacy and the world's last hope.
The Overworld theme, appropriately named "Adventure", gives players a foreshadowing of epic the journey is going to be.
Hero's Challenge — the first time a Dragon Quest final boss has had two themes — plays when you finally use the Sphere of Light to weaken Zoma so he can be defeated for the first time in history. (Symphonic from DQ 9.)
The Overture makes its appearance here, but another special note of just when it appears is required: it's only after we watch the hero's mother apparently die in childbirth, the crushing silence broken by his newborn cries that the overture begins. This is how you add atmosphere to a game.
As is usual for the series, the Overworld theme, in this case called Toward the Horizon, is excellent.
An Orchestrated Remix of the Overture, where the Dragon Quest dev team and Level 5 show off their fancy new engine to the tune of an Orchestrated remix of the song all while a pair of birds fly for miles using the in game engine and no load times. Epic. Just Epic.
Everything. Just everything. Featuring remixes of nearly the entire Dragon Quest OST library, all done in glorious symphonic remixes. They went all out for the series' glorious return to the west, and it shows.