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When the titular character tries to stake Dracula in the heart.
Dracula: (looks at stake, smiles) Hello, Gabriel.
Despite being a "gypsy" princess who has spent most of the movie needing to be saved by Van Helsing, when she's been kidnapped by the last of Dracula's brides and is about to be sucked, Kate Beckinsale redeems herself with this exchange:
Aleera: Anna, my love. It is your blood that shall keep me beautiful. What do you think of that? [laughs triumphantly, then screams as Anna catches a silver stake and impales her through the chest] Anna Valerious: I think if you're going to kill somebody, kill them! Don't stand around talking about it!