These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Accidental Aesop: Can be both good or bad; Gilda and Diamond Tiara were sent home because of their attitude and bullying, seemingly providing the aesop that bullying is bad. However, Sweetie Belle is sent home for making one mistake that anypony could have made and wasn't even entirely her fault, which may be telling readers that you have to do everything perfectly, or everyone will hate you.
The fact that the unicorns are treated as useless due to not having their magic, Pegasus wings, or earth pony power seems like saying if you lose whatever you're good at, you will become completely useless to everybody around you.
Complete Monster: The only even remotely nice thing Gilda does is reluctantly congratulate Apple Bloom for pulling their team to victory in one challenge. Every other moment, she acts just as bad as in her own episode in the show, if not more so. Subverted when she returns in chapter 14, as she's become a bit nicer; more of a Jerk with a Heart of Gold.
Crazy Awesome: Pinkie Pie, like in the series, is insane but awesome.
Squick: The cupcake-eating challenge. Including Trixie cupcakes, made out of Trixie's fur, hoof clippings, and saliva. Almost everypony reacts this way, and even more when Twilight eats a Trixie cupcake and likes it.