These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
If you fail to rescue Queen Aino when she is raised out of the water, you get a cutscene where she flops around a bit, then dies.
This, like any other Game Over in the game, is followed up by a cutscene where the camera pulls away from a dead Jen in the (now darkened) hospital room, followed by a fade to black. The sad music doesn't help matters, either.