The Drowning Pit sequence. Mickey struggling to swim upwards even though he's chained up, noticeably exhaling when he can't hold his breath, and finally fainting. The air bubbles that escapes his mouth even form his famous head silhouette. Also counts as Mood Whiplash with the sign gag: "You must be THIS TALL to survive the dungeon."
Although the sign gag is Played for Laughs, Mickey's face when he sees this basically says "That's it, I am going to die."
A gag with tombstones for the trio in Pete's lair, each one with a carved skull shaped like the character's heads on top of each one.