This film contains examples of:
- Acceptable Targets: Desmond Lovely and Gareth Nice, the fundamentalist religious protestors with devastating speech impediments (Tourettes and a heavy stutter respectively). Played with, in that although their speech problems are milked for plenty of humour, of the religious protestors they're clearly a lot nicer and more open-minded than the obnoxiously self-righteous Andrew Thorogood.
- Big Lipped Alligator Moment: One minute, John Cleese and Michael Palin are in Michael's study having an argument about the debate. The next, marionette versions of Cleese and Palin are having a slow-mo Hong Kong-cinema style sword fight over cheap mock-ups of the rooftops of London armed with a sword and a lightsaber respectively. Even by the standards of Monty Python, that comes right out of left-field. And then, in classic Python style, it just ends.
- Ho Yay: The ongoing jokes surrounding the portrayal of Michael's wife as Terry Jones in drag; potentially John's declaration of love for Michael. Also Alan Dick kissing Harry Balls in celebration.
- Refuge in Audacity: In keeping with subject matter, there's lots of this about. It basically begins with Jesus Christ declaring that the Bible is 'largely made-up' and then power-farting in the face of one of his disciples when he objects, and then goes from there.
- Unpopular Popular Character: The portrayal of John Cleese.
- Vindicated by History: Life of Brian is now regarded as one of the best British films ever made, an acknowledgement made just before the credits.