- The 1966 Batman: The Movie featured a team-up between Joker, Penguin, Riddler and Catwoman.
- Several of the TV episodes of that series featured team-ups between various villains, they did no better or worse than usual against Batman.
- Batman Forever has Riddler and Two Face team up, while Batman & Robin has Mr. Freeze and Poison Ivy team up (whether Bane is a villain or a henchman, you pick). In the latter's case Ivy is manipulating Freeze, which doesn't work out for well for her when he finds out. Batman Returns deconstructs this trope, showing Catwoman and the Penguin working together for all of five minutes before breaking it off.
- Batman Begins has the Scarecrow initially working with Falcone, and unwittingly working for Ra's Al Ghul. The Dark Knight is borderline, as the Joker creates the second villain but never truly works with him; however, the Joker does initially work with the mob. In The Dark Knight Rises, Selina Kyle being blackmailed into providing some assistance to Bane is borderline given she becomes more of an Anti Heroine later and is the one who kills Bane at the end... but Talia Al Ghul being the woman behind the man is a definite case.
- Kamen Rider Den-O: Climax Deka features the Imagin Negataros forms an "evil syndicate", and to strengthen their numbers, he recruited Fangire into his cause. In turn, this necessitates the arrival of the Fangires' main enemy, Kamen Rider Kiva, to come to the aid of Den-O and his allies.
- All of the Marvel Cinematic Universe films feature this:
- Iron Man features the Ten Rings terrorist group teaming up with Obadiah Stane/Iron Monger.
- The Incredible Hulk (2008) has General Ross and Emil Blonsky, before Blonsky forces Samuel Stern to turn him into the Abomination, forcing Ross to team up with the heroes to beat him.
- Iron Man 2 has Whiplash team up with Justin Hammer, although their alliance is short-lived.
- Thor gets twisty. Loki manages to convince Laufey of Jotunheim that they're working towards the same end but winds up betraying him so that he can justify an attack against Jotunheim.
- Captain America: The First Avenger has the Red Skull and Arnim Zola.
- The Avengers (2012) has Loki and the Chitauri soldiers, later revealed to be an army on loan to him by Thanos.
- Iron Man 3 has the Mandarin and Aldrich Killian, along with Eric Savin. Subverted, as it turns out that "the Mandarin" is just a British actor who is used as a face by Killian, and the real Mandarin isn't happy with someone stealing his name.
- Thor: The Dark World features Malekith the Accursed and Algrim, who becomes Kurse.
- Captain America: The Winter Soldier has the eponymous Winter Soldier, [[Brock Rumlow Crossbones]], and a computerized Arnim Zola all working for Alexander Pierce and HYDRA. Batroc the Leaper appears to be working with them as well, though he ends up being just a Red Herring.
- Guardians of the Galaxy (2014) has Ronan the Accuser allying with Nebula, who's acting as an emissary of Thanos. They both turn on him once getting the Infinity Stone.
- Avengers: Age of Ultron has Ultron team up with Quicksilver and Scarlet Witch, who work with him under the impression that he can help them get revenge on Tony Stark. They defect to the heroes' side once they find out what Ultron's ultimate goal is. Ulysses Klaue also appears as an Arms Dealer who sells vibranium to Ultron.
- Ant-Man comes the closest to averting this, but near the end it's revealed that Darren Cross has been knowingly associating with undercover members of HYDRA and is preparing to sell them the Yellowjacket and Ant-Man suits. More subtly one of the HYRDA mooks is revealed by a tattoo to be associated with the Ten Rings organization.
- Captain America: Civil War inverts this: the most teaming up that the main antagonist Helmut Zemo does is through coercion with mostly unwilling partners. The main team-ups are done by the opposing groups of heroes, and which side is the more antagonistic one is left up to the viewer.
- Doctor Strange (2016) features the sorcerer Kaecilius and his zealots attempting to contact the cosmic being Dormammu in order for him to bring about the engulfment of Earth into the Dark Dimension.
- Guardians of the Galaxy Vol. 2 has Nebula team up with a group of rogue Ravagers to overthrow Yondu, and said Ravagers also have dealings with Ayesha of the Sovereigns to help her get revenge on the Guardians. None of them however team up with the film's main villain Ego the Living Planet, and Nebula teams up with the Guardians to help take him down.
- Spider-Man: Homecoming features the Vulture employing the Tinkerer, Darter, Montana, and the Shocker, and selling weapons to the Scorpion. The Prowler attempts to buy weapons for him as well, but later expresses his discomfort with the Vulture's weapons and helps Spider-Man find him. It's also implied that the Prowler at one point had dealings with the Scorpion before the events of the film.
- Thor: Ragnarok has Hela team up with Skurge to take over Asgard, while on the planet Sakaar, Scrapper 142 (who is later revealed to be Valkyrie) collects prisoners for the Grandmaster. Both underlings end up rebelling and joining the heroes. Loki naturally switches sides a few times before ending the movie on Thor's side. The movie also features an interesting variation on this trope: to stop Hela, Loki unleashes Surtur to bring about Ragnarok on Asgard and destroy her, in a strange instance of one antagonist teaming up with another to intentionally help the heroes.
- Black Panther (2018) has Killmonger teaming up with Klaue to steal some Vibranium and sell it on the black market. However, it later turns out that Killmonger was planning all along to betray Klaue, so that he can present his corpse as a present to Wakanda, to buy his way into an audience with the elders and lay his claim to the throne.
- Avengers: Infinity War features Thanos teaming up with the Black Order (referred to in-film as the "Children of Thanos") in order to retrieve the Infinity Stones. Subverted with Loki, who attempts to re-form his alliance with Thanos from the first Avengers film; it ends up just being a ruse so that he can get close enough to Thanos and stab him. Thanos ends up seeing through Loki's deception and kills him instead.
- Ant-Man and the Wasp has the Ghost and Bill Foster, but neither of them could be considered outright villains by the end of the movie. The film's other major antagonist, Sonny Burch, has contacts within the FBI but beyond that does not team up with any of the other villains.
- Captain Marvel (2019) features Yon-Rogg teaming up with Ronan the Accuser to take down Carol near the end of the film, though Ronan does it under the impression that they're wiping out a Skrull invasion on Planet C-53 (a.k.a. Earth).
- Avengers: Endgame has Thanos once again teaming up with the Black Order. As the movie is a Time Travel film that has the heroes going back to the events of The Avengers, Thor: The Dark World and Guardians of the Galaxy, then the team-ups featured in those films are technically implied in Endgame, with Thanos from 2014 at one point discussing his team-up with Ronan and recognizing the Avengers from 2023 due to his team-up with Loki in the first Avengers film.
- Spider-Man: Far From Home features Quentin Beck recruiting a crew of disgruntled ex-Stark Industries to create the Elementals and Mysterio.
- Black Widow (2021) has Dreykov and his Black Widow assasins, as well as Taskmaster. Yelena Belova and Melina Vostokoff work with him as well before their defections.
- Spider-Man:
- The Amazing Spider-Man 2 has a Villain Team-Up between Harry Osborn/the Goblin and Max Dillon/Electro. The alliance is shaky (since Dillon doesn't have any sentiment for Harry due to his background with Oscorp; Harry brokers the team-up by saying both of them were shafted by Oscorp) but Dillon's desire to strike back at Oscorp and Spider-Man is enough to get them working together. At the end, Harry is working to compile a team of empowered individuals to eliminate Spider-Man, which heavily suggests that he wants to form the Sinister Six.
- Spider-Man 3 has a team up with Sandman and Venom, with New Goblin just sort of hanging around.
- Superman II has Lex Luthor initiate a team up with the 3 Kryptonians, but ends up with the short end of the stick very quickly.
- Super Sentai crossover movies typically have the current villain group team up with the remnants of the previous villain group.
- X-Men: The Last Stand had Magneto convince Jean Grey in Phoenix form to work together; sadly, this was because Jean didn't trust Charles Xavier and killed him while Magneto watched.
- Glass (2019), the third in M. Night Shyamalan's superhero trilogy, has Mister Glass (the villain of Unbreakable) and the Horde (the villain of Split) incarcerated in the same psychiatric institution, along with David Dunn. Glass convinces the Horde to team up, break out of the institution and cause havoc, forcing Dunn to break out too.Mister Glass: That sounds like the bad guys teaming up.
