- Acting for Two:
- Jeff Bergman voices Bugs Bunny, Daffy Duck, Foghorn Leghorn, and Pepé Le Pew.
- Jess Harnell voices both Tosh and Pete Puma.
- Ariane Price voices both the French woman and the perfume girl.
- Descended Creator: Co-writer and co-voice director Rachel Ramras voices Lola Bunny.
- Directed by Cast Member: In addition to playing Lola, Rachel Ramras co-directed the voice actors.
- Written by Cast Member: Ramras also co-wrote the script and co-wrote the song "Girl, You're Fabulous".
- Invisible Advertising: Cartoon Network did squat when advertising the film's premiere. This is downplayed however, as the film had already been on home video for months by this point.
- The Other Darrin: Some recasting of characters from The Looney Tunes Show is done here...
- Co-writer Rachel Ramras voices Lola Bunny, doing an imitation of Kristen Wiig, who voiced Lola on The Looney Tunes Show.
- Pete Puma is voiced by Jess Harnell, having previously been voiced by John Kassir.
- Damon Jones voices Marvin the Martian, having previously provided Marvin's rapping voice in The Looney Tunes Show but not the speaking voice (done by Eric Bauza).
- Sequel Gap: Was released 12 years after the previous Looney Tunes film Looney Tunes: Back in Action.
- Role Reprise: In the main voice cast, Rachel Ramras (Lola) and Jess Harnell (Pete Puma) are the only actors who haven't played their characters before.
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