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Tearjerker: The Curse of the Were-Rabbit
Wallace's reaction to learning that he's the were-rabbit everyone's been hunting. He starts desperately trying to fix a device that might reverse the process, only to find that his brain is pretty much "rabbit mush". He ends up breaking down, sobbing "Oh Gromit, I don't want to be a giant rabbit!"
When he transforms again, the sheer panic in his voice as he desperately begs Gromit to help him.
"You've gotta help me, Gromit! Hide me! Anything! Before it's too late!"
Victor locks Gromit in a cage while he goes after the Were-Rabbit (Wallace). Gromit desperately tries to get out for a few minutes. Then he slumps, and, even though there's no sound and we can't see his face, it's clear that he's crying.
Gromit crying over the seemingly dead Were-Rabbit Wallace. Granted he comes back to life, but it was almost to the point of seeing a character many of us were introduced to at childhood die.