The Littlest Cancer Patient Shirley in the second season of Vandread. Made worse by her friendship with Bart and hisgoing into an Unstoppable Rage to save an her on a planet from the Harvesters only to find out she died during the battle from her disease. A double whammy comes from the show cutting to her trying to hold on so she can see Bart again.]]
The Red Shirt Army at the end of the first season. Hibiki meets them, they get some depth and they aren't going let their planet get harvested, and the harvest fleet they were trying to stop proceeds to effortlessly tear them apart while Hibiki is powerless to help them.