- Harley being dumped and kicked out by the Joker, though she gradually gets "emancipated", and surely for the best.
- Harley bringing her roller derby friends margaritas, only to overhear them talking about how she's inevitably going to run back to Joker because she (presumably) can't stand on her own. She runs off in drunken tears.
- While it's played for laughs, the moment when Harley's sandwich is splattered across the road before she can take a bite is still pretty unfortunate considering she used the last of her money to pay for it.
- The life of Mr. Keo's daughter is not spared by Black Mask after the latter had a sudden change of mind. His reason for doing it (he got grossed out by a snot bubble she had when she was crying in utter terror), and his method of killing her (cutting her face off), made it all the more horrible.
- Harley confides in Dinah about her and Joker's split with what starts as an amusing drunken ramble but turns into a teary, bittersweet confession, talking about the role of a harlequin, reflecting how lost both women are feeling.Harley: Do you know what a harlequin is? A harlequin's role is to serve. It's nothing without a master. No one gives two shits who we are, beyond that.
- Cassandra Cain avoiding her foster parents, who seem to be in an Awful Wedded Life. Considering that her foster father yells that he doesn't even want the girl in his house, it's unsurprising that she decides to run away with Harley instead.
- Helena Bertinelli saw her whole family being gunned down when she was still a child.
- We learn that Dinah Lance's mother, the first Black Canary, died at some point. According to Dinah, her body was left in the streets with the police she had previously aided doing nothing to help.
- The explosion in Harley's apartment seemingly kills Harley's hyena pet Bruce, the only living being she loved after breaking up with the Joker (though it's ultimately a Disney Death and they are reunited at the end).
- Harley is near sobbing when Doc, the one guy she always counted on as a friend and a near-saint, gives her up for a big payday and shrugs it off as "it's just business."
- Roman cruelly humiliating a woman in his club for thinking she was laughing at him. It even makes Dinah shed tears.
