This is based on opinion. Please don't list it on a work's trope example list.
Nightmare Fuel / The Last Temptation of Christ
A terrifying film in general. Throughout the movie there appear several calm moments but have an underlying sense of eeriness. However, there's several disturbing scenes.
One of them being a crucifixion scene in the beginning where a man is crucified for sedition. His screaming in the scene is just unbearable, and the look of guilt in Jesus' eyes when the blood from the man's ankles sprays on his face is just heartbreaking.
Jesus pulling his heart out. The scene is very ominously lit and has eerie yet epic music accompanying it.
Jesus healing the lepers after the aforementioned scene is made even more eerie is the music playing over the scene.