Darth Vader and Luke Skywalker teaming up against The Emperor and reconciling their differences, just before the former's death.
Superman and Batman reconciling.
At the end of Lego Marvel after Magneto destroyed the Statue of Liberty, it is being rebuilt and Captain America gives Agent Coulson a hug in the background.
Even after the events of Lego Marvel, in which of course they were antagonists, Loki and Thor team up in the bonus level "Bro-tunheim".
The Thing carrying Human Torch after the Green Goblin boss fight.
Despite (or even because of) the fact that the Hulk remains the Hulk through the entire game on account of Rule of Funny, seeing that his friendship with Tony Stark is just as strong as in the movies brings a smile to one's face.
After Hawkeye and Widow are injured during the Red Skull level, Coulson tells Fury they're fine and promises to bring them back safely.